The estimated one-repetition maximum (1RM) bench press and NFL-225 (225-lb or 102-kg) repetition test are commonly used to assess upper-body muscular strength and endurance among football players. However, little research has been focused on the relationship of these tests to playing status. Therefore, the purpose of this study was to determine if significant relationships exist between these tests and playing status in Division I football athletes. Archival data from 31 NCAA Division I football players (age: 20.1±1.4 yrs., height: 188.07 ± 5.93 cm, body mass: 112.4 ± 19.5 kg) on the 1RM Bench press test, NFL-225 test and playing status were utilized for this analysis. A one-way ANOVA was used to detect any differences in 1RM and NFL-225 performance between skill groups: big (linemen), medium (linebackers, quarterbacks, tight ends) and small (receivers, backs, and corners) (p < 0.05). Playing status (starters vs. non-starters) were compared within position groups. A point bi-serial correlation was then utilized to examine the relationship in test performance between groups, as well as between starters and non-starters. Significant differences were discovered in NFL-225 test performance between big and small skill groups. Moderate-to-strong relationships between playing status and performance on the 1RM bench press (r = .660) and the NFL-225 test (r = .685) for the big skills group. The results of this study suggest that playing status and upper-body strength and endurance are strongly related for the big skills position group.